top of page
< Back

Calcium and α-tocopherol suppress cured-meat promotion of chemically induced colon carcinogenesis in rats and reduce associated biomarkers in human volunteers

Pierre, Fabrice H. F.; Martin, Océane C. B.; Santarelli, Raphaelle L.; Taché, Sylviane; Naud, Nathalie; Guéraud, Françoise; Audebert, Marc; Dupuy, Jacques; Meunier, Nathalie; Attaix, Didier; Vendeuvre, Jean-Luc; Mirvish, Sidney S.; Kuhnle, Gunter C. G.; Cano, Noel; Corpet, Denis E.

Date Published:





Extra Links:



PMID: 24025632 PMCID: PMC3798078


BACKGROUND: Processed meat intake has been associated with increased colorectal cancer risk. We have shown that cured meat promotes carcinogen-induced preneoplastic lesions and increases specific biomarkers in the colon of rats. OBJECTIVES: We investigated whether cured meat modulates biomarkers of cancer risk in human volunteers and whether specific agents can suppress cured meat-induced preneoplastic lesions in rats and associated biomarkers in rats and humans. DESIGN: Six additives (calcium carbonate, inulin, rutin, carnosol, α-tocopherol, and trisodium pyrophosphate) were added to cured meat given to groups of rats for 14 d, and fecal biomarkers were measured. On the basis of these results, calcium and tocopherol were kept for the following additional experiments: cured meat, with or without calcium or tocopherol, was given to dimethylhydrazine-initiated rats (47% meat diet for 100 d) and to human volunteers in a crossover study (180 g/d for 4 d). Rat colons were scored for mucin-depleted foci, putative precancer lesions. Biomarkers of nitrosation, lipoperoxidation, and cytotoxicity were measured in the urine and feces of rats and volunteers. RESULTS: Cured meat increased nitroso compounds and lipoperoxidation in human stools (both P < 0.05). Calcium normalized both biomarkers in rats and human feces, whereas tocopherol only decreased nitro compounds in rats and lipoperoxidation in feces of volunteers (all P

Automatic Tags

Female; Humans; Male; Adult; Aged; Middle Aged; Cholesterol; Diet; Analysis of Variance; Biomarkers; Single-Blind Method; Calcium, Dietary; Blood Glucose; Rats; C-Reactive Protein; Cross-Over Studies; Healthy Volunteers; Human; Dietary Fiber; Creatinine; Meat; Acetylcysteine; Vitamin E; Carcinogenesis; Lipid Peroxidation; Carcinogens; France; Colorectal Neoplasms; Feces; Colon; alpha-Tocopherol; Thiobarbituric Acid Reactive Substances; Randomized Controlled Trials; Rats, Inbred F344; Inulin; Middle Age; Animal Studies; Crossover Design; Data Analysis Software; Descriptive Statistics; Funding Source; In Vivo Studies; Random Assignment; Biological Markers; T-Tests; Calcium Carbonate; Meat Products; Feces -- Analysis; Post Hoc Analysis; Colorectal Neoplasms -- Prevention and Control; Colorectal Neoplasms -- Risk Factors; Dimethylhydrazines; Diphosphates; Diterpenes, Abietane; Rutin; Colon -- Analysis; Diet, Antineoplastic; Food Additives -- Pharmacodynamics; Histological Techniques; Single-Blind Studies; Sodium Compounds; Wilcoxon Signed Rank Test

bottom of page