< Back

Ruminant fat intake improves gut microbiota, serum inflammatory parameter and fatty acid profile in tissues of Wistar rats

de Brito Medeiros, Larissa; Alves, Susana Paula Almeida; de Bessa, Rui José Branquinho; Soares, Juliana Késsia Barbosa; Costa, Camila Neves Meireles; de Souza Aquino, Jailane; Guerra, Gerlane Coelho Bernardo; de Souza Araújo, Daline Fernandes; Toscano, Lydiane Tavares; Silva, Alexandre Sérgio; Alves, Adriano Francisco; Lemos, Mateus Lacerda Pereira; de Araujo, Wydemberg José; de Medeiros, Ariosvaldo Nunes; de Oliveira, Celso José Bruno; de Cassia Ramos do Egypto Queiroga, Rita

Date Published:

Publication:

DOI:

URL:

PMID:

Extra Links:

September 23, 2021

10.1038/s41598-021-98248-6

Click to open url

Bandiera_abtest: a Cc_license_type: cc_by Cg_type: Nature Research Journals Number: 1 Primary_atype: Research Publisher: Nature Publishing Group Subject_term: Cardiology;Health care;Risk factors Subject_term_id: cardiology;health-care;risk-factors

Abstract:

This study tested the hypothesis that naturally and industrially produced trans-fatty acids can exert distinct effects on metabolic parameters and on gut microbiota of rats. Wistar rats were randomized into three groups according to the diet: CONT-control, with 5% soybean oil and normal amount of fat; HVF-20% of hydrogenated vegetable fat (industrial); and RUM-20% of ruminant fat (natural). After 53 days of treatment, serum biochemical markers, fatty acid composition of liver, heart and adipose tissue, histology and hepatic oxidative parameters, as well as gut microbiota composition were evaluated. HVF diet intake reduced triglycerides (≈ 39.39%) and VLDL levels (≈ 39.49%). Trans-fatty acids levels in all tissue were higher in HVF group. However, RUM diet intake elevated amounts of anti-inflammatory cytokine IL-10 (≈ 14.7%) compared to CONT, but not to HVF. Furthermore, RUM intake led to higher concentrations of stearic acid and conjugated linoleic acid in all tissue; this particular diet was associated with a hepatoprotective effect. The microbial gut communities were significantly different among the groups. Our results show that ruminant fat reversed the hepatic steatosis normally caused by high fat diets, which may be related to the remodelling of the gut microbiota and its anti-inflammatory potential.