Dietary lysozyme supplementation contributes to enhanced intestinal functions and gut microflora of piglets

Lysozyme plays a significant role in defense against bacterial pathogens and in regulating the interactions between gut microbiota and host immune systems. Here, the effects of dietary lysozyme on the intestinal development, immunity, and colonic microbiota of piglets were comprehensively evaluated. Twenty-four seven-day-old piglets from Landrace × Yorkshire sows (n = 8 per group) received no supplementation (group A, the control), 0.5 g kg-1 lysozyme (group B), or 1.0 g kg-1 lysozyme (group C). After the 14-day treatment, piglets supplemented with 1.0 g kg-1 lysozyme had higher average weaning weight, jejunal villus height (VH), and ileal lymphocyte counts than those in the control groups (P < 0.005). Serum total protein and albumin were significantly up-regulated (P < 0.005) and immunoglobulin G tended to increase in the 0.5 g kg-1 lysozyme group (P = 0.065). Bacteroidetes, Proteobacteria, and Fibrobacteres all showed a significant increase in relative abundance after lysozyme treatment at the highest dosage (P